Radioactive yeast fractions derived from C14 labeled pyruvate and acetate.

A study of the utilization of CH&140COOH and CH&1400H by yeast. has revealed significant differences in the rat.e and manner of disappearance of these substances from the growth medium, as well as in yeast growth (1). Since tagging of the two compounds in the manner indicated would render all pyruvate molecules equivalent. t.o acetate after decarboxylation and oxidation, the extent of their equivalence in anabolic processes can be readily evaluated. The results presented herein point to relatively large variations in the conversion of these substrates to fat,ty acids, humin, and amino acids.